欢迎光临,现在是   设为首页   加入收藏
文章摘要
引用本文:
不同病原性相关分子模式对大黄鱼(Larimichthys crocea)头肾巨噬细胞免疫功能的影响
Effects of pathogen-associated molecular patterns on the immunologic function of the head kidney macrophages from large yellow croaker (Larimichthys crocea)
投稿时间:2020-03-12  修订日期:2020-04-04
DOI:
中文关键词: 大黄鱼、巨噬细胞、病原相关分子模式、非特异性免疫、细胞因子
英文关键词: Larimichthys crocea, macrophages, pathogen-associated molecular patterns, non-specific immunity, cellular inflammatory factors
基金项目:海南省自然科学基金;海南省南海名家青年项目
作者单位E-mail
刘肖岑 海南大学 2079619511@qq.com 
周永灿 海南大学  
曹贞洁 海南大学  
孙云 海南大学 syshui207@126.com 
摘要点击次数: 58
全文下载次数: 0
中文摘要:
      为研究不同病原性相关分子模式(PAMP)对大黄鱼头肾巨噬细胞免疫功能的影响,本研究分别将脂多糖(lipopolysaccharide,LPS)、肽聚糖(peptidoglycan,PGN)、脂磷壁酸(lipoteichoic acid,LTA)、胞壁酰二肽(muramyl dipeptide,MDP)与提取的大黄鱼头肾巨噬细胞进行孵育,测定大黄鱼头肾巨噬细胞的呼吸爆发活性、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、溶菌酶(LZM)活性和免疫相关细胞炎症因子(TNFα、IL1β、IL8、IL10、CXCL9、MYD88、TLR3和TLR8)的表达情况。实验结果显示:(1)LPS、PGN、LTA、MDP均能显著性提高大黄鱼头肾巨噬细胞的呼吸爆发活性和LZM活性,同时显著降低SOD和CAT两种抗氧化酶的活性。(2)在LPS和LTA刺激下,8种免疫相关细胞炎症因子TNFα,IL1β,IL8,IL10,CXCL9,MYD88,TLR3和TLR8均呈显著性上调趋势;在PGN和MDP刺激下,除IL8、CXCL9外,其余6种免疫相关细胞炎症因子均显著性上调。研究结果表明,4种病原相关分子模式LPS、PGN、LTA、MDP均能显著影响大黄鱼头肾巨噬细胞的非特异性免疫应答能力和免疫相关细胞炎症因子的表达。
英文摘要:
      In order to study the effects of pathogen-associated molecular patterns on the immunologic function of the head kidney macrophages (HKMs) from large yellow croaker (Larimichthys crocea), the head kidney macrophages from L. crocea were extracted and then incubated with lipopolysaccharide (LPS), peptidoglycan (PGN), lipoteichoic acid (LTA), muramyl dipeptide (MDP), respectively. After that, the respiratory burst activity, antioxidant enzyme (SOD & CAT) activity, lysozyme (LZM) activity and the expression of immune-related cellular inflammatory factors (TNFα, IL1β, IL8, IL10, CXCL9, MYD88, TLR3, and TLR8) of HKMs from L. crocea were detected. The results showed that LPS, PGN, LTA, and MDP could increase the respiratory burst activity and LZM activity of HKMs from L. crocea, while the two antioxidant enzymes SOD and CAT activities were reduced. Meanwhile, the expression level of TNFα, IL1β, IL8, IL10, CXCL9, MYD88, TLR3, and TLR8 were significantly up-regulated after LPS and LTA stimulation. Furthermore, other six immune-related cellular inflammatory factors were significantly up-regulated after PGN and MDP stimulation, except for IL8 and CXCL9. These results of this study suggested that these four pathogen-associated molecular patterns, LPS, PGN, LTA and MDP, could affect the non-specific immunity and the expression of immune-related cellular inflammatory factors of HKMs from L. crocea.
View Fulltext   查看/发表评论  下载PDF阅读器
关闭