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引用本文:潘学峰,张夏莲.黄姜花组培快繁技术[J].热带生物学报,2013,4(2):189-193.
黄姜花组培快繁技术
Rapid Propagation of Hedychium flavum Roxb.
  
DOI:
中文关键词: 黄姜花  块茎  组培快繁
英文关键词: Hedychium flavum  tuber  rapid propagation  tissue culture
基金项目:国家科技基础条件平台建设子项目(2005DKA21006)
作者单位
潘学峰 1.海南大学 农学院海南 海口570228 
张夏莲 2.海口市秀英区东山镇人民政府 海南 海口 571154 
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中文摘要:
      以黄姜花的笋芽为外植体,研究了外植体的消毒、丛生芽的增殖及生根培养等关键步骤的配方。结果表明:1)利用ρ=1 g·L-1的升汞对外植体进行消毒的最佳时间为12 min;2)黄姜花丛生芽增殖的较优配方为:3/4 MS+6-BA 3.5 mg·L-1+水解酪蛋白1 000 mg·L-1+蔗糖30 g·L-1,继代周期25 d,增殖倍数为5.83;3)1/2 MS+NAA 0.5 mg·L-1的生根培养基效果最好,平均生根数达9.17条,平均根长2.73㎝,植株健壮;4)将试管苗移裁在V河砂︰V表土=1︰1的基质中,25 d后成活率可达91.67%,植株生长良好。
英文摘要:
      Shoots newly sprouting from underground rhizome of Hedychium flavum Roxb. ere used as explants for rapid propagation. The explants were sterilized, induced into clustered shoots, generated into plantlets and rooted on different media, and then transferred onto the mixed substrates of sand and surface soil for culture. The explants sterilized with HgCl2(1 g·L-1) for 12 min gave best result. The optimal medium for proliferation of clustered shoots was 3/4MS + 6-BA 3.5 mg·L-1 + CH 1 000 mg·L-1 + sugar 30 g·L-1. On this medium clustered shoots were subcultured for 25 days as a cycle at a proliferation coefficient of 5.83. The plantlets were best rooted on the rooting medium of 1/2MS+NAA 0.5 mg·L-1, producing upto 9.17 roots/plantlet with an average root length of 2.73 cm, and the rooted plantlets grew robust and 5.43 cm tall by average. The rooted plantlets transferred onto the substrate of river sand 50% + surface soil 50% (V︰V) for culture gave 91.67% survival after 25 days of transfer and grew well.
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