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引用本文:曹玉鑫,花龙,赵天龙,李明容,夏志辉.Xa21全生育期表达载体的构建[J].热带生物学报,2013,4(2):133-137.
Xa21全生育期表达载体的构建
Construction of Vector Expressing Xa21 during Whole Growth Stage
  
DOI:
中文关键词: Xa21  蔗糖合酶基因启动子  白叶枯病  水稻  全生育期
英文关键词: Xa21  promoter of rice sucrose synthase gene 1  bacterial leaf blight  rice  whole growth stage
基金项目:国家自然科学基金(31071173);海南省教育厅高校科研资助项目(Hjkj2012-12);海南大学科研启动经费(KYQD1001)
作者单位
曹玉鑫 海南大学 农学院海南 海口 570228 
花龙 海南大学 农学院海南 海口 570228 
赵天龙 海南大学 农学院海南 海口 570228 
李明容 海南大学 农学院海南 海口 570228 
夏志辉 海南大学 农学院海南 海口 570228 
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中文摘要:
      利用PCR技术,以水稻品种明恢63基因组DNA与携Xa21完整基因组片段的载体pDBXa21作为模板,分别克隆了RSuS1启动子(RSP)、Xa21的开放阅读框至终止子的片段,并构建了RSP驱动水稻广谱抗白叶枯病基因Xa21的转基因表达载体。实时荧光定量PCR技术证实,水稻蔗糖合酶基因1(rice sucrose synthase gene 1,RSuS1)在水稻种子中低表达,在叶鞘与叶中全生育期高效表达。
英文摘要:
      In this study, the results of realtime PCR of rice sucrose synthase gene 1(RSuS1) showed that RSuS1 was highly expressed in sheath and leaf during whole growth stage. The promoter of RSuS1 was amplified from genomic DNA template of an indica rice variety “Minghui 63” , then the DNA fragment of Xa21 including open reading frame and terminator was amplified from vector pDBXa21 carrying completely genomic Xa21 fragment. In order to breed transgenic rice with resistance to bacterial leaf blight during whole growth stage and without risk of comestible safety, we constructed an expression vector that Xa21 was driven by RSP.
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