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引用本文:潘梅,符瑞侃,王景飞,吕德任,黄赛.花叶艳山姜茎尖离体快繁技术[J].热带生物学报,2012,3(3):267-270.
花叶艳山姜茎尖离体快繁技术
Rapid propagation of Alpinia zerumbet cv. Vaniegata
  
DOI:
中文关键词: 花叶艳山姜  茎尖  快速繁殖
英文关键词: Alpinia zerumbet cv. vaniegata  shoot tip  rapid propagation
基金项目:
作者单位
潘梅 海南省农业科学院 园林花卉研究所海南 海口 571100 
符瑞侃 海南省农业科学院 园林花卉研究所海南 海口 571100 
王景飞 海南省农业科学院 园林花卉研究所海南 海口 571100 
吕德任 海南省农业科学院 园林花卉研究所海南 海口 571100 
黄赛 海南省农业科学院 园林花卉研究所海南 海口 571100 
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中文摘要:
      以花叶艳山姜(Alpinia zerumbet cv. vaniegata)的茎尖作为外植体,研究了花叶艳山姜的离体快繁技术。结果表明:花叶艳山姜茎尖接种在MS+BA 3.0 mg·L-1+NAA 0.2 mg·L-1 +白糖30 g·L-1的培养基上,30 d后长出幼芽;丛生芽在MS + 6-BA 2.0~2.5 mg·L-1+NAA 0.2 mg·L-1 +白糖30 g·L-1的培养基上,增殖效果最佳,增殖倍数达4.0,芽生长正常,且色泽浓绿;芽苗在1/2MS+NAA 1.0~1.5 mg·L-1 +白糖30 g·L-1的培养基上,生根效果较好,根系粗壮,生根率为93.0%;组培苗移栽在V河沙︰V珍珠岩︰V表土=1︰1︰1的混合基质中,成活率可达70.1 %。
英文摘要:
      Shoot tips were used as explants to mass propagate Alpinia zerumbet cv. vaniegata. The shoot tips were cultured on the MS medium at the presence of BA 3.0 mg·L-1+NAA 0.2 mg·L-1+ sugar 30 mg·L-1 for 30 days and produced adventitious shoots continuously. These shoots were transferred onto the MS medium supplemented with 6-BA 2.0—2.5 mg·L-1+NAA 0.2 mg·L-1 + sugar 30 mg·L-1 for further culture and were well proliferated with a proliferation coefficient of 4.0. The multiplied shoots grew normal with dark green color and were rooted well with stout roots on the 1/2MS medium supplemented with NAA 1.0—1.5 mg·L-1 with a rooting rate of 93.0%. The rooted plantlets of A.zerumbet cv. vaniegata were transplanted in the mixed substrate (river sand: perlite: surface soil =1∶1∶1), and survived upto 70.1%.
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